In 1905, at the age of 27, Dr. Grigorov made the discovery, for which he is best known. In the microbiological laboratory of Professor Masole in Geneva, he discovered that a certain strain of bacillus is the true cause for the existence of natural yoghurt. In recognition the strain was called by the scientific community Lactobacillus bulgaricus.
His further research carried out in many institutes worldwide proved that Bulgarian yogurt helps in the treatment of various diseases and conditions like infections, otic-rhino-laryngeal diseases, tuberculosis, stomach and intestine conditions, ulcers, some gynecologic diseases, fatigue, etc. These prophylactic and curative properties of Bulgarian yogurt are due to the rich vitamin content including B1, B2, C, A, D, E, PP, B12, as well as lactose, proteins, and other important stimulating substances.
Apart from the discovery of Lactobacillus bulgaricus, Dr. Grigorov made a major contribution to the creation of an anti-tuberculosis vaccine. On 20 December 1906, in Paris in issue No104 of the “La Presse Médicale” medical journal, he published his scientific report “The Anti-tuberculosis vaccine”, which informed the scientific community about the results of his research into the application of penicillin fungi for the treatment of tuberculosis. After the publication, the scientific community expressed a serious interest in Dr. Grigorov’s vaccine. Through his scientific experiments “in-vitro” and “in-vivo” on lab animals and later on human patients, Dr. Grigorov clearly demonstrated and described the healing effect of penicillin fungi in the treatment of tuberculosis.